DMEM/F12 medium is based on DMEM medium, with more abundant nutrients in F12 medium added. It contains a variety of trace elements and is widely used in the culture of various mammalian cells. At the same time, DMEM/F12 medium is often used as the basis for the development of serum-free medium, and is also suitable for the culture of mammalian cells under low serum content and clonal density culture.
HEPES is an excellent biological buffer with no toxic effect on cells. The medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.
This product contains a variety of ingredients such as amino acids, vitamins, and inorganic salts required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
