G-418 (Geneticin) is an aminoglycoside antibiotic. In molecular genetic experiments, it is the most commonly used resistance screening agent for stable transfection. G-418 (Geneticin) can interfere with the function of 80S ribosomes and affect protein synthesis in eukaryotic cells. It is mainly used for the selection of mammalian, plant or yeast cells. G-418 (Geneticin) is toxic to bacteria, yeast, higher plants, mammalian cells, protozoa and worms. Its resistance gene is a dominant phenotype and is located on both Tn601 (903) and Tn5 transposons. Although this gene is derived from bacteria, it can also be expressed in eukaryotic cells. Introducing resistance genes into cells can cause resistance to G-418, allowing cells to grow in culture medium containing G-418. This selection property of G418 has been widely used in gene transfer, gene knockout, resistance screening and transgenic animals.
Precautions
1. This product is sterilized by 0.1μm filtration;
2. When using this product, pay attention to aseptic operation to avoid contamination;
3. This product is a concentrated solution, please dilute it as needed before use;
4. It should not be placed at room temperature or stored at 4℃ for a long time;
5. Thaw at 2℃～8℃, shake well before use, avoid repeated freezing and thawing, and it is recommended to be packaged and frozen when the amount is small;
6. This product is only used for scientific research or further research, not for diagnosis and treatment.
