Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. DMEM medium was originally designed with a glucose content of 1000 mg/L (low sugar type), and later developed with a glucose content of 4500 mg/L (high sugar type). It is now widely used in the culture of various cells.
DMEM low-glucose medium is more suitable for the culture of adherent cells with slow metabolism.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for the culture of various types of cells; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.