Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. DMEM medium was originally designed with a glucose content of 1000 mg/L (low sugar type), and later developed with a glucose content of 4500 mg/L (high sugar type). It is now widely used in the culture of various cells.
DMEM high-sugar medium is commonly used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, DNA-transfected transformed cells, various primary virus host cells, single cell culture and vaccine production, such as using CHO cells to express EPO and produce hepatitis B vaccine.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not freeze-thaw;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
