Ham's F-12 Nutrient Mixture was designed by Ham in 1969 based on Ham's F-10 Nutrient Mixture and was originally used for serum-free culture of CHO cells. Ham's F-12 is often used as a basic culture medium for serum-free culture. When the serum content is low, it is particularly suitable for single cell culture and cloning culture. After adding serum, it is also widely used in the culture of cancer cells and primary cells, such as rat hepatocytes, rat prostate epithelial cells, chondrocytes, rat myoblasts, chicken embryonic cells, etc. In addition, after mixing Ham's F-12 with DMEM in equal volumes, the resulting DMEM/F12 culture medium is more nutritious and more widely used.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.