The buffer system of Leibovitz's L-15 medium is composed of phosphate and free base amino acids, which replaces the traditional sodium bicarbonate buffer system. At the same time, the use of galactose and sodium pyruvate instead of glucose can prevent the formation of acidic metabolic byproducts, help maintain the stability of the pH of the culture medium, and is suitable for cell culture in non-CO2 balanced environments. Leibovitz's L-15 medium is suitable for the culture of monkey kidney cells and HEP-2, the isolation of primary cells (such as embryonic and adult tissues), the culture of various viruses, and the culture of neurons.
This product contains a variety of ingredients such as amino acids, vitamins, and inorganic salts required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.