MEM medium (Minimum Essential Medium) is also called the minimum essential medium, minimum basic medium or low-limit Eagle medium. It was developed by Harry Eagle on the basis of Eagle basic medium (BEM). It is the most basic and widely applicable medium and one of the most commonly used medium in animal cell culture. MEM medium contains only 12 essential amino acids, glutamine and 8 vitamins. It has simple ingredients and can be used for various suspended and adherent mammalian cells, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, fibroblasts and primary rat astrocytes.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
