McCoy's 5A medium was designed by Thomas McCoy and others in 1959. It was originally used for the culture of Novikoff Hepatoma cells. McCoy's 5A medium is a modified Basal Medium 5A. Unlike other mediums, McCoy's 5A contains reduced glutathione, bacterial peptone and high concentration of glucose. McCoy's 5A is widely used for the culture of various types of primary cells, such as bone marrow, skin, gums, kidneys, spleen, lungs, rat embryos, omentum, etc. In addition, McCoy's 5A medium is also used for tissue biopsy culture, cell line establishment, and the culture of some lymphocytes and cells that are difficult to culture.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for the culture of various types of cells; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
