RPMI 1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Farrow, New York, USA. RPMI is a type of cell culture medium developed by the institute, and 1640 is the code name of the culture medium. RPMI 1640 is an improved McCoy's 5A medium that uses a bicarbonate buffer system. Unlike most mammalian cell culture media, its typical pH 8 formula is different. RPMI 1640 medium was originally designed for lymphocyte culture. It is now widely used in the culture of various normal cells and cancer cells, especially suspension cells, and is one of the most widely used culture media.
RPMI 1640 medium without glucose can adjust the glucose concentration at will according to research needs, which is convenient and fast.
HEPES is an excellent biological buffer that is non-toxic to cells. The culture medium with HEPES added can maintain a constant pH range for a long time, which can effectively prevent the adverse effects of large pH fluctuations in the culture medium on cell growth.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
