MEM α (Minimum Essential Medium α) is a modified MEM medium. Compared with MEM medium, MEM α medium is more nutritious. On the basis of MEM, NEAA, sodium pyruvate, zinc sulfate, VB12, biotin, ascorbic acid and other ingredients are added. It is widely used in mammalian cell culture and transfection of DHFR-negative cells. MEM α can be used for various suspended and adherent mammalian cells, including keratinocytes, primary rat astrocytes and human melanoma cells.
MEM α medium without nucleosides and deoxynucleosides is often used as a screening medium for DG44 and other DHFR-deficient cells.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, please do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
