Serum-free supplement for MSCs
A type of pluripotent stem cell with broad application potential has been extensively researched and used in fields such as regenerative medicine and immunomodulation. To meet the needs of research and clinical applications, GinoBio has launched the MSC Serum-Free Expansion Kit, supporting your research and applications.
Product Overview
GENOMBIO offers two product formats for the MSC Serum-Free Expansion Kit to meet diverse experimental needs:
MSC Serum-Free Expansion Kit: A two-component complete medium suitable for the culture of primary and passaged MSCs derived from sources such as umbilical cord, adipose tissue, and dental pulp.
MSC Serum-Free Basal Medium: An optimized basal medium designed to be used with platelet lysate or fetal bovine serum from other brands for enhanced efficiency.
Product Features
Efficiency: The optimized serum-free medium requires a lower usage amount and offers higher cell culture efficiency, while preserving the cell characteristics of serum-free cultures, which helps maintain the stability of the MSC therapeutic effect.
Versatility: Suitable for the culture of MSCs derived from sources such as umbilical cord, adipose tissue, and dental pulp. It supports both primary culture and long-term passaging of MSCs.
Safety: Serum-free and xeno-free, with raw materials undergoing strict testing to ensure safe and reliable composition.
Note: Contains human-derived proteins, such as albumin, and is not suitable for applications strictly requiring no human-derived proteins. It is appropriate for the early expansion stage of exosome production, with a medium change at the exosome harvest stage.
Product Specifications
Basic Quality Control Indicators
Physicochemical:
Appearance inspection
pH (6.9-7.6)
Sterility (negative)
Endotoxin (less than 1 EU/mL)
Osmolality (280-320 mOsm/kg)
Biological Activity Testing:
Cell culture testing
Storage and Transportation
MSC Serum-Free Basal Medium:
Store at 2-8°C, with a shelf life of 12 months
Transport with cold packs
MSC Serum-Free Supplement Components:
Store frozen at -20°C or below; avoid repeated freeze-thaw cycles
Do not exceed three freeze-thaw cycles, with a shelf life of 12 months
Transport with dry ice
Product Usage
Complete Medium:
Store the prepared complete medium at 2-8°C and use within 4 weeks
Supplement Components:
Avoid repeated freeze-thaw cycles; do not exceed three freeze-thaw cycles
Can be aliquoted for use
Product Data
Cell Morphology
Typical fibroblast-like morphology of MSCs, with fewer polygonal cells and better cell viability compared to hPL and fetal bovine serum.
Comparison of Cell Expansion with Competing Products
Continuous passaging tests demonstrate a significant advantage.
Umbilical Cord MSC Marker Analysis
Umbilical cord MSCs cultured up to the 10th passage were subjected to marker analysis, confirming their MSC biological characteristics: CD90, CD105, and CD73 showed a positive rate of over 95%, while CD45, CD19, CD34, CD11b, and HLA-DR showed a positive rate of less than 2%.
MSC Differentiation Testing
Umbilical cord MSC adipogenic differentiation test: On the 14th day of differentiation, MSCs were observed to have differentiated into adipocytes, with distinct lipid droplets visible inside the cells after Oil Red O staining.
Adipose-derived MSC adipogenic differentiation test: By the 14th day of differentiation, nearly all MSCs had differentiated into adipocytes, with distinct lipid droplets observed within the cells after Oil Red O staining.
Umbilical cord MSC osteogenic differentiation test: On the 14th day of differentiation, most MSCs were observed to have differentiated into osteoblasts. Alizarin Red staining showed calcium deposits occupying part of the well bottom.
Adipose-derived MSC osteogenic differentiation test: By the 21st day of differentiation, most MSCs were observed to have differentiated into osteoblasts. Alizarin Red staining revealed calcium deposits occupying the vast majority of the well bottom.
Umbilical cord MSC chondrogenic differentiation test: By the 22nd day of differentiation, MSCs were observed to have differentiated into chondrocytes. Alcian Blue staining revealed distinct blue granules within the cells, indicative of cartilage formation.
Adipose-derived MSC chondrogenic differentiation test: On the 22nd day of differentiation, MSCs were observed to have differentiated into chondrocytes. Alcian Blue staining showed distinct blue granules within the cells, indicating successful chondrogenesis.