Each cell has its own specific markers, so the cells can be specifically identified. The basic principle of immunofluorescence identification is: through the specific binding of fluorescent antibodies to cell surface antigens, after washing to remove free fluorescent antibodies, observe under a fluorescent microscope, and bright specific fluorescence can be seen on a dark background, and the antigenic substances in the specimen can be identified.
Experimental steps:
1. Fixation
2. Elimination of endogenous peroxidase
3. PBS washing
4. Blocking
5. Incubation of primary antibody
6. PBS washing
7. Incubation of secondary antibody
8. PBS washing
9. DAPI counterstaining
10. Sealing
11. Fluorescence microscope observation
Result example:
