PI, i.e. propidium iodide, can bind to DNA and RNA in cells. After RNA is digested with RNase, the fluorescence intensity of PI bound to DNA detected by flow cytometry directly reflects the amount of DNA content in cells. Due to the different DNA content in each phase of the cell cycle, the G0/G1 phase of normal cells usually has the DNA content of diploid cells (2N), while the G2/M phase has the DNA content of tetraploid cells (4N), and the DNA content of the S phase is between diploid and tetraploid. Therefore, when the intracellular DNA content is detected by flow cytometry PI staining, the phases of the cell cycle can be divided into G0/G1 phase, S phase and G2/M phase, and the percentage of each phase can be calculated by special software.
Experimental steps:
1. Cell plating
2. Cell synchronization treatment
3. Sample collection
4. On-machine detection
5. Synchronized data analysis
Result case:
