RPMI 1640 was developed by Moore et al. in 1967 at the Roswell Park Memorial Institute (RPMI) in Farrow, New York, USA. RPMI is a type of cell culture medium developed by the institute, and 1640 is the code name of the culture medium. RPMI 1640 is an improved McCoy's 5A medium that uses a bicarbonate buffer system. Unlike most mammalian cell culture media, its typical pH 8 formula is different. RPMI 1640 medium was originally designed for lymphocyte culture. It is now widely used in the culture of various normal cells and cancer cells, especially suspension cells. It is one of the most widely used culture media.
Phenol red is used as a pH indicator in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. It is red at pH 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium without phenol red. Phenol red will interfere with the detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas will interfere with sodium-potassium balance.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
