Balanced Salt Solution (BSS), also called Physiological Solution, is a solution that combines the buffering capacity of a buffer, the isotonicity of a physiological saline solution, and the nutritional supply characteristics of a culture medium. It has the functions of maintaining osmotic pressure, keeping pH stable, and providing simple nutrition, and can meet the basic needs of survival and metabolism of tissues, organs, or cells in in vitro experiments. Some balanced salt solutions also add a small amount of phenol red to indicate changes in the pH of the solution.
Earle's Balanced Salt Solution, the full name of which is Earle's Balanced Salt Solution (EBSS), is one of the most commonly used phosphate buffers. Its main components include NaCl, KCl, Na2HPO4, NaHCO3, etc. Earle's Balanced Salt Solution is often used for rinsing tissue blocks, washing cells, transporting cells or tissues, preparing other reagents, and as a diluent for cell counting. Earle's Balanced Salt Solution containing calcium and magnesium is often used as a transport fluid or for preparing other reagents.
Precautions
1. This product is ready-to-use and can be used directly after 0.1μm filtration sterilization. No dilution or filtration sterilization or other treatment is required before use;
2. When using this product, pay attention to aseptic operation to avoid contamination;
3. Since the solution contains calcium and magnesium ions, a small amount of precipitation is easy to produce. Generally, the precipitation can be dissolved after warm bath; if there is a lot of precipitation and it cannot be dissolved, please give up using it;
4. This product is only used for scientific research or further research, not for diagnosis and treatment.
