Balanced Salt Solution (BSS), also called Physiological Solution, is a solution that combines the buffering capacity of a buffer, the isotonicity of a physiological saline solution, and the nutritional supply characteristics of a culture medium. It has the functions of maintaining osmotic pressure, keeping pH stable, and providing simple nutrition, and can meet the basic needs of survival and metabolism of tissues, organs, or cells in in vitro experiments. Some balanced salt solutions also add a small amount of phenol red to indicate changes in the pH of the solution.
D-Hank's Balanced Salt Solution is a Hank's Balanced Salt Solution (HBSS) that does not contain calcium or magnesium ions. It is one of the commonly used phosphate buffers in cell separation or culture. Its main components include NaCl, KCl, KH2PO4, Na2HPO4, NaHCO3, and glucose. D-Hank's Balanced Salt Solution is often used for washing before cell passage or tissue dissociation, preparation of cell dissociation solution, transportation of cells or tissues, and cell counting dilution solution.
Precautions
1. This product is ready-to-use and can be used directly after 0.1μm filtration sterilization. No dilution or filtration sterilization or other treatment is required before use;
2. When using this product, pay attention to aseptic operation to avoid contamination;
3. Due to the low NaHCO3 content of D-Hank's balanced salt solution, it cannot be used in a 5% CO2 environment. If placed in a CO2 culture phase, the solution will quickly become acidic, so please pay attention when using it;
4. This product is only used for scientific research or further research, not for diagnosis and treatment.
