Balanced Salt Solution (BSS), also called Physiological Solution, is a solution that combines the buffering capacity of a buffer, the isotonicity of a physiological saline solution, and the nutritional supply characteristics of a culture medium. It has the functions of maintaining osmotic pressure, keeping pH stable, and providing simple nutrition, and can meet the basic needs of survival and metabolism of tissues, organs, or cells in in vitro experiments. Some balanced salt solutions also add a small amount of phenol red to indicate changes in the pH of the solution.
Hank's Balanced Salt Solution (HBSS) is one of the commonly used phosphate buffers in cell separation or culture. Its main components include NaCl, KCl, KH2PO4, Na2HPO4, NaHCO3, CaCl2, MgCl2, MgSO4, and glucose. Hank's Balanced Salt Solution is often used for rinsing tissue blocks, washing cells, transporting cells or tissues, preparing other reagents, and as a diluent for cell counting.
1. This product is ready-to-use and can be used directly after 0.1μm filtration sterilization. No dilution or filtration sterilization or other treatment is required before use;
2. When using this product, pay attention to aseptic operation to avoid contamination;
3. Since Hank's balanced salt solution contains calcium and magnesium ions, it is easy to produce a small amount of precipitation. Generally, the precipitation can be dissolved after warm bath; if there is a lot of precipitation and it cannot be dissolved, please give up using it;
4. Since Hank's balanced salt solution has a low NaHCO3 content, it cannot be used in a 5% CO2 environment. If it is placed in a CO2 culture phase, the solution will quickly become acidic. Be careful when using it;
5. This product is only used for scientific research or further research, not for diagnosis and treatment.
