Place the Transwell chamber in the culture plate. The chamber is called the upper chamber, and the culture plate is called the lower chamber. The upper chamber contains the upper culture medium, and the lower chamber contains the lower culture medium. The upper and lower culture mediums are separated by a polycarbonate membrane. Plant the cells in the upper chamber. Since the polycarbonate membrane is permeable, the components in the lower culture medium can affect the cells in the upper chamber, so that the effects of the components in the lower culture medium on cell migration and movement can be studied.
Experimental steps:
1. Incubate cell culture reagents and Transwell at 37℃
2. Collect logarithmic phase cells, suspend cells, and count
3. Add serum-containing culture medium to the lower chamber and cell suspension to the upper chamber for culture
4. Methanol fixation
5. Crystal violet staining
6. Neutral resin sealing
7. Statistical results
Result case:
