Cell culture is one of the most commonly used methods in biological and medical research, and can be divided into primary culture and subculture. Primary culture is to obtain cells directly from the organism for culture. Since the cells have just been separated from the living tissue, they are closer to the living state in the organism.
This method can provide a powerful means for studying the growth, metabolism, and reproduction of biological cells, and also create conditions for subsequent subculture. This method can also directly serve clinical practice.
Experimental steps:
1. Preparation of reagents and consumables
2. Take fresh animal tissue
3. Enzyme digestion
4. Filter and separate cells
5. Add culture medium, 37℃, 5% CO2 culture
6. Subculture
7. Cryopreservation
8. Resuscitation
Result case:
Rat Schwann cells
Rat cardiomyocytes
Human aortic valvular interstitial cells (HAVIC)
