Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. The glucose content of DMEM medium was originally designed to be 1000 mg/L (low sugar type), and later developed to be 4500 mg/L (high sugar type). It is now widely used in the culture of various cells.
DMEM high-sugar medium is commonly used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, DNA-transfected transformed cells, various primary virus host cells, single cell culture and vaccine production, such as using CHO cells to express EPO and produce hepatitis B vaccine.
Phenol red is used as a pH indicator in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. It is red at pH values of 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium without phenol red. Phenol red can interfere with flow cytometry. In addition, the presence of phenol red in some serum-free culture medium formulas can interfere with sodium-potassium balance.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
