Dulbecco's Modified Eagle Medium (DMEM) is developed on the basis of MEM medium. Compared with MEM medium, the amino acid content is increased by 2 times, the vitamin content is increased by 4 times, and non-essential amino acids, trace iron ions and sodium pyruvate are also added. DMEM medium was originally designed with a glucose content of 1000 mg/L (low sugar type), and later developed with a glucose content of 4500 mg/L (high sugar type). It is now widely used in the culture of various cells.
DMEM high-sugar medium is commonly used in fast-growing, low-adhesion cells, myeloma cells of hybridomas, cloned cells, DNA-transfected transformed cells, various primary virus host cells, single cell culture and vaccine production, such as using CHO cells to express EPO and produce hepatitis B vaccine.
Penicillin-streptomycin mixture is also commonly known as "double antibiotic". It is the most commonly used antibiotic in in vitro culture to prevent microbial contamination. Among them, penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria; while streptomycin can bind to the 30S subunit of bacterial ribosomes and inhibit the synthesis of bacterial proteins. It is effective against both Gram-negative and Gram-positive bacteria, but is particularly effective against Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the best use effect of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
