IMDM (Iscove’s Modified Dulbecco Medium) is Iscove’s modified DMEM medium, which was designed by Guilber and Iscove in 1976 to culture erythroid precursor cells and macrophages. IMDM medium is based on DMEM medium and adds selenium, HEPES, sodium pyruvate, additional amino acids and vitamins, and replaces ferric nitrate with potassium nitrate. It is very nutritious and very suitable for rapid proliferation and high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, LPS-stimulated B cells, bone marrow hematopoietic cells, T lymphocytes and various hybridoma cells), but also can be used as the base liquid for some unique serum-free culture media.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product has been sterilized by filtration. When using it, please pay attention to aseptic operation to avoid contamination;
2. To maintain the effect of this product, please do not freeze-thaw it;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
