IMDM (Iscove’s Modified Dulbecco Medium) is Iscove’s modified DMEM medium, which was designed by Guilber and Iscove in 1976 for culturing erythroid precursor cells and macrophages. IMDM medium is based on DMEM medium and adds selenium, HEPES, sodium pyruvate, additional amino acids and vitamins, and replaces ferric nitrate with potassium nitrate. It is very nutritious and very suitable for rapid proliferation and high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, LPS-stimulated B cells, bone marrow hematopoietic cells, T lymphocytes and various hybridoma cells), but also can be used as the base liquid for some unique serum-free culture media.
Phenol red is used as a pH indicator in the culture medium to continuously monitor the pH of the culture medium. At low pH values, phenol red makes the culture medium yellow, while at higher pH values, it makes the culture medium purple. It is red at pH values of 7.2-7.4, which is most suitable for cell culture. However, phenol red also has some disadvantages. Studies have shown that phenol red can simulate the effects of steroid hormones (especially estrogen). Therefore, when using estrogen-sensitive cells (such as breast tissue), it is best to use a culture medium without phenol red. Phenol red will interfere with the detection during flow cytometry analysis. In addition, the presence of phenol red in some serum-free culture medium formulas will interfere with sodium-potassium balance.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the effect of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
