IMDM (Iscove’s Modified Dulbecco Medium) is Iscove’s modified DMEM medium, which was designed by Guilber and Iscove in 1976 to culture erythrocyte precursor cells and macrophages. IMDM medium is based on DMEM medium and adds selenium, HEPES, sodium pyruvate, additional amino acids and vitamins, and replaces ferric nitrate with potassium nitrate. It is very nutritious and very suitable for rapid proliferation and high-density cell culture. IMDM medium can not only culture cells with special nutritional requirements (such as mouse B lymphocytes, LPS-stimulated B cells, bone marrow hematopoietic cells, T lymphocytes, and various hybridoma cells), but also can be used as the base liquid for some unique serum-free culture media.
Penicillin-streptomycin mixture is also commonly known as "double antibiotic". It is the most commonly used antibiotic in in vitro culture to prevent microbial contamination. Among them, penicillin can interfere with the synthesis of bacterial cell walls and is particularly effective against Gram-positive bacteria; while streptomycin can bind to the 30S subunit of bacterial ribosomes and inhibit the synthesis of bacterial proteins. It is effective against both Gram-negative and Gram-positive bacteria, but is particularly effective against Gram-negative bacteria. The combined use of penicillin and streptomycin can prevent most bacterial contamination.
This product contains a variety of ingredients such as amino acids, vitamins, inorganic salts, etc. required for various types of cell culture; but it does not contain proteins, lipids or any growth factors; therefore, this product needs to be used with serum or serum-free additives.
Precautions
1. This product is sterilized by filtration. When using it, pay attention to aseptic operation to avoid contamination;
2. To maintain the effect of this product, do not perform freeze-thaw treatment;
3. This product is only used for scientific research or further research, not for diagnosis and treatment.
