Balanced Salt Solution (BSS), also called Physiological Solution, is a combination of the buffering capacity of a buffer, the isotonicity of a physiological saline solution, and the nutrient supply characteristics of a culture medium. It has the functions of maintaining osmotic pressure, keeping pH stable, and providing simple nutrition, and can meet the basic needs of survival and metabolism of tissues, organs, or cells in in vitro experiments. Some balanced salt solutions also add a small amount of phenol red to indicate changes in the pH of the solution.
Phosphate-Buffered Saline (PBS) is the most widely used balanced salt solution in biochemical research. Its main components are Na2HPO4, KH2PO4, NaCl, and KCl. PBS buffer is often used for rinsing tissue blocks, washing cells, transporting cells or tissues, preparing other reagents, and as a diluent for cell counting.
Precautions
1. This product is ready-to-use and can be used directly after 0.1μm filtration sterilization. No dilution or filtration sterilization or other treatment is required before use;
2. When using this product, pay attention to aseptic operation to avoid contamination;
3. This product is 10 times the concentration of PBS. Please dilute it before use as needed;
4. 10×PBS may produce a small amount of precipitation at low temperature. Generally, the precipitation can be dissolved after warm bath; if there is a lot of precipitation and it cannot be dissolved, please give up using it.
5. This product is only used for scientific research or further research, not for diagnosis and treatment;
